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WG3 - Gene expression; transcriptomics; proteomics


Convenor: Keith Edwards, Bristol

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Transcriptomics platforms, proteomics and metabolomics


Many or most traits of interest that control the sustainability and value of the crop, ranging from biotic and abiotic stress resistance to grain quality and plant architecture, are derived from the action of multiple, interacting genes. Technologies to identify differentially expressed genes and to analyse gene expression levels on a genomic scale are available. In particular, efforts have led to the development of common barley and wheat microarrays on the Affymetrix platform.

Proteomics tools are relatively underdeveloped, compared with transcriptome techniques. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) setups are available as are more sophisticated tools. These include tandem mass spectrometry (MS-MS), two-dimensional liquid chromatography (2D-LC), also coupled to MS-MS, and automated MALDI-TOF systems. Likewise, analysis of the metabolome remains a major development target for such important systems as developing endosperm.

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