MonoGram 2017

This meeting is in the past: but this page is updated with summaries, presentations, prize winners... so come back often!

GARNish 27: GARNET's take on the event!

The meeting was held in the iconic Wills Memorial Building in the centre of Bristol, from the 4th-6th April 2017 with the conference dinner on board the world famous SS Great Britain.

The Monogram Network meeting is the annual get together for the small-grain cereal and grass research community. Academics, commercial scientists and plant breeders meet to hear about the latest advances, exchange ideas and discuss collaboration.

We are pleased to announce the following keynote speakers for Monogram 2017:

Poster Prize winners

PhD poster prize winner – Carus John-Bejai (University of Nottingham & KWS UK)

Originally from Trinidad and Tobago, Carus was exposed to plant science from a young age as both of his parents are involved in agriculture and forestry. His PhD project is a joint venture between KWS UK and The University of Nottingham and began in the autumn of 2013 and will be completed by the end of 2017. His research entailed ascertaining how much variation exists for floral traits within European germplasm and how this variation, if any, can be used towards the development of efficient male parental lines for hybrid breeding schemes. They first set out to assess a number of phenotyping methods under controlled conditions in order to determine how accurately they can phenotype for these traits but also whether or not they would be suitable for screening a large number of lines in field trials. Appropriate methods for assessing anther extrusion, anther length and flowering pattern were subsequently applied to a panel of 111 winter wheat genotypes in two field trials. An incredible amount of variation was observed within our small panel, even amongst locally adapted varieties. Further we observed that phenotypic values across two years were strongly correlated. These two observations support the feasibility of breeding for more efficient males. They hope that the methods applied in our study, in conjunction to the development of tools for marker assisted selection, will aid in realising this goal.

Post-doc poster prize winner – Nicole Schatlowski (NIAB)

Originally from Germany, Nicole is working at NIAB, Cambridge on a shrunken endosperm mutant in barley, called lys3<, which has unusually large embryos. With this very interesting combination she is hoping to learn more about the developmental control of organ size, and also would like to exploit the nutritional benefits of the large embryos. However, the genetic bases underlying the Lys3 mutation has not been identified yet. Published information placed Lys3 on the long arm of chromosome 5 and our initial mapping results confirmed this location. Nicole is currently fine-mapping Lys3 using two approaches: selection of homozygous recombinant lines followed by phenotyping and also sequencing of flow sorted chromosomes of independent lys3 mutant alleles to look for a gene mutated in all of them. So far she has narrowed down the Lys3 candidate region to ca. 130 genes (based on the latest IPK genome sequencing data). In addition, she noticed that isolated lys3 embryos seem to have a high regeneration and transformation potential whereas the WT and most barley cultivars cannot readily be transformed. Currently the team are exploring whether this can be used to generate transformable elite cultivars for the research community.

Programme PDF

Tuesday 4th April

Wills Memorial Building, Queens Rd, Bristol BS8 1RJ

9.00-10.00 Registration and coffee

10.00-1.00 MonoGram Cereal Bioinformatics Workshop (Chaired by Cristobal Uauy) *Sponsored by the Bristol Centre for Agricultural Innovation (BCAI)*

  • Clemence Marshal (JIC): Update on genome assemblies available for wheat.
  • Luca Venturini (EI): From fragment to whole: progress in wheat genome annotation
  • Cristobal Uauy (JIC) : Update on pan-genome. Download PDF
  • Olu Shorinola (JIC) : Integrated use of Wheat Genome Resources: a case study Download PDF
  • Guy Naamati (EBI) : Ensembl plants and wheat: update from a busy year
  • Sergio Contrino (Camb.) : Araport Download PDF
  • Kelly Houston (JHI) : Barley Update Download PDF
  • Tim Langdon(Aber) : Oat update
  • Paul Wilkinson (Bristol) : CerealsDB- expansion of resources and data integration
  • Jemima Brinton: (JIC) :, Download PDF
  • Ajit Singh (Rothamsted) : KnetMiner: Gene discovery through data mining and interactive visualization

1.00-2.00 Lunch

2.00 Welcome address: Prof. Keith Edwards 

2.15-5.50pm Quality & Nutrition
Chaired by Rowan Mitchell (Rothamsted Research) and Sofia Kourmpetli (Cranfield University)

  • 2:15-2:45 Keynote speaker: Odd-Arne Olsen (Norwegian University of Life Sciences, Norway) *Sponsored by RAGT* : Cereal endosperm; lessons learnt from molecular biology, cell biology, genomics and transcriptomics analysis. Download PDF
  • 2:45-3:05 Till Pellny (Rothamsted Research): Development of wheat with low-viscosity grain extracts using novel alleles of IRX9 orthologue TaGT43_2. Download PDF
  • 3:05-3:25 Bill Thomas (JHI): MPROMALT: Improving the malting quality of UK winter barley. Download PDF
  • 3:25-3:45 Oluwaseyi Shorinola (JIC): Waking up too early: TaMKK3-A underlies the major 4AL pre-harvest sprouting resistance locus, Phs-A1, in UK and global germplasm Download PDF
  • 3.45-4.05 Flash presentations I (Chaired by Dusty Vyas, LGC Genomics)
  • 4.05-4.30 Coffee break
  • 4.30-4.50 Kay Trafford (NIAB): Production of wheat lacking B-type starch granules. Download PDF
  • 4.50-5.10 Sophie Harrington (JIC): Moving towards a better understanding of wheat senescence using novel genetic resources and protein structure homology to model species. Download PDF
  • 5.10-5.30 Mark D. Wilkinson (Rothamsted Research): The Gsp-1 genes encode the wheat arabinogalactan peptide . Download PDF
  • 5.30-5:50 Flash Presentations II (Chaired by Gabriella Everett, KWS) 

Welcome drinks – local ciders and cheeses *Sponsored by Bayer*

6-8pm Poster session and judging *Sponsored by Syngenta*

Tower Tours (optional)

Wednesday 5th April

Wills Memorial Building, Queens Rd, Bristol BS8 1RJ

9.00-11:30 From Arabidopsis to Cereals and Back Again *Sponsored by GARNet*
Chaired by Geraint Parry (GARNet co-ordinator, University of Cardiff) and Zoe Wilson (University of Nottingham). Download PDF

  • 9.00-9.30 Keynote speaker: Ian Henderson (University of Cambridge: Recombination and chromatin landscapes in plant genomes.
  • 9.30-9.50 Jose Fernandez (University of Nottingham): Developing a Cereal Fertility Pipeline (CerFip) for wheat and barley.
  • 9.50-10.10 Candida Nibau (Aberystwyth University). Arabidopsis as a model to study the function of Ph1-like protein kinases in chromosome pairing and recombination. Download PDF
  • 10.10-10.30 Alistair Hetherington (University of Bristol): Manipulation of transpiration to improve seed Fe and Zn. Download PDF
  • 10.30-10.50 Coffee break
  • 10.50-11.10 Sarah McKim (UoD at the JHI): Moving on Up – Internode growth in cereals. Download PDF
  • 11.10-11.20 Stephanie Smith (University of Cambridge) - Translating mechanisms of biological nitrogen response from Brachypodium to cereal crops. Download PDF
  • 11.20-11.30 Syabira Yusoff (University of Leicester) - Brachypodium as a valuable resource for investigation cereal grain biology and evolution. Download PDF
  • 11.30-11.40 Lukas Wittern (University of Cambridge) - Genetic analysis of the effect of circadian clock genes on yield components in wheat (Triticum aestivum).
  • 11:30-11.40 Jemima Brinton (John Innes Centre) - Two tightly-linked genes controlling grain length underlie a major grain weight QTL in polyploid wheat. Download PDF

11:40-12:30 MECEA award winners
Chaired by Sarah McKim (UoD at the JHI) 

  • 11.40-12.05 PhD: Jemima Brinton(John Innes Centre) - Two tightly-linked genes controlling grain length underlie a major grain weight QTL in polyploid wheat. Download PDF
  • 12.05-12.30 Post-doc: Cara Griffiths, (Rothamsted Research)

12:30-1:30 Lunch and posters *Sponsored by Limagrain*

1:30-3:30 Genomic technology
Chaired by Kelly Houston (JHI) and Chris Burt (RAGT)

  • 1.30-2.00 Keynote speaker: Susanne Dreisigacker (CIMMYT, Mexico) *Sponsored by RAGT*: Advances in Genomics-assisted Breeding in the CIMMYT Global Wheat Program. Download PDF
  • 2.00-2.20 Amanda Burridge (University of Bristol): Array-based single nucleotide polymorphic makers for use in targeted genotyping by sequencing in hexaploid wheat. Download PDF
  • 2.20-2.40 Katherine Steele (Bangor University): Increasing the accessibility of KASP technology for rice improvement. Download PDF
  • 2.40-3.00 William Jackson (The Sainsbury Laboratory): The barley ‘nibblerome’: defining the set of NB-LRR-type R-genes from a diverse collection of barley. Download PDF
  • 3.00-3.20 Julio Isidro Sánchez (University College Dublin): Design of training population for genomic prediction. Download PDF

30-4.00 Coffee Break 

4.00-6.00 New projects and areas
Chaired by Scott Boden (JIC) and Harriet Trewin (BBSRC)

  • 4.00-4.10 Harriet Trewin (BBSRC) : BBSRC Funding opportunities.
  • 4.10-4.30 Invited speaker: Keith Edwards (University of Bristol) : Use of a modified CRISPR/Cas9 construct to simultaneously mutagenise multiple genes in hexaploid wheat. Download PDF
  • 4.30-4.50 Invited speaker: Ben Trevaskis (CSIRO, Australia) : A genome-to-phenome platform for Australian wheat research. Download PDF
  • 4.50-5.05 Harriet R. Benbow (University College Dublin): The genetics of host and non-host resistance to Septoria Tritici Blotch. Download PDF
  • 5.05-5.20 Ryan Joynson (Earlham Institute) : Using Genetic Approaches to Exploit Phenotypic Variation in Photosynthetic Efficiency to Increase Wheat Yield. Download PDF
  • 5.20-5.35 Rainer Melzer (University College Dublin): Employing the ABC model of flower development to generate wheat varieties with enhanced disease resistance. Download PDF
  • 5.35-5.50 Philippa Borrill (JIC): Gene regulatory networks reveal key players in the regulation of senescence in wheat. Download PDF
  • 5.50-6.05 Simon Hammann (University of Bristol) : Lipidome profiling of cereals and biomarker characterization for the identification of cereal processing in archaeological pottery

7pm Conference dinner on board the SS Great Britain

*Sponsored by LGC Genomics*

Followed by

After Party in the Hayward Saloon

*Sponsored by DSV*

Thursday 6th April

9:30-12:30 Phenotyping technology and applications
Chaired by Alison Bentley (NIAB) and Donal O’Sullivan (University of Reading)

  • Using MAGIC Download PDF
  • 9:30-10:00 Keynote Speaker: Tobias Würschum (University of Hohenheim, Germany): Integration of precision phenotyping and genomics for cereal breeding. Download PDF
  • 10:00-10:30 Invited speaker: Lee Hickey (University of Queensland, Australia): High-throughput phenotyping to dissect the genetics of cereal root architecture.
  • 10:30-11:00 Coffee break
  • 11:00-11:20 Stéphanie Swarbreck (University of Cambridge): Nutrient availability to wheat roots modulates their response to the presence of blackgrass.
  • 11:20-11:40 Samer Amer (University of Reading): A novel pipeline for dissecting genetic architecture of wheat yield responses: linking semi-automated phenotyping and Bayesian network modelling. Download PDF
  • 11:40-12:00 Anyela Camargo (University of East Anglia) : Genetic mapping of quantitative trait loci for longitudinal traits in a wheat MAGIC mapping population. Download PDF
  • 12:00-12:20 John Doonan (Aberystwyth University): Exploiting Biomedical Imaging approaches for non-destructive grain phenotyping. Download PDF
  • 12:20-12:30 Yeorgia Argirou (NIAB) Using MAGIC to dissect the genetics of below- and above-ground phenotypes in wheat. Download PDF

12:30-1:30 Lunch

1:30-3:30 Crop-pathogen interactions
Chaired by Simon Berry (Limagrain) and Sacha Allen (University of Bristol)

  • 1.30-1.55 Invited speaker: Dr Darren Soanes (University of Exeter): Comparative Genomics of Wheat Blast Fungus. Download PDF
  • 1.55-2.20 Josh Hegarty (University of California, USA): Validation and characterization of two QTL for adult plant resistance to stripe rust on wheat chromosome arms 6DS (Yr77) and 6BS (Yr78). Download PDF
  • 2.20-2.40 James Gerard Hehir (Teagasc): Field evaluations of the synthetically derived T. aestivum cv. Stigg highlights the importance of the pre-symptomatic phase in supporting strong partial resistance against Zymoseptoria tritici. Download PDF
  • 2.40-3.00 Emma Wallington (NIAB) : Engineering wheat for take-all resistance. Download PDF
  • 3.00-3.20 Beant Singh (Rothamsted Research): Characterization of bird cherry-oat aphid (Rhopalosiphum padi L.) behaviour and host preference in relation to resistant and susceptible Watkins wheat lines.
  • 3.20-3.40 Kar-Chun Tan (Curtin University, Australia): Breeding for resistance to necrotrophic fungal diseases of wheat. Download PDF

See you next year!