Cereal Transformation Laboratories

The following pages contain the results of a survey (conducted during summer 2008) of the current status of cereal transformation in Europe. I restricted my survey to public-sector laboratories. I sent 50 questionnaires and received 34 replies from 32 separate institutions in13 different countries. The information will be updated regularly. If you are active in the cereal transformation field and not included in these results, please , contact Huw Jones (BBSRC)

Groups Working on Common Wheat
Groups Working on Durum Wheat
Groups Working on Barley
Groups Working on Oats
Groups Working on Rye
Groups Working on Tritordium
Groups Working on Triticale
Groups Working on Grasses
Groups Working on Brachypodium
Groups Working on Rice
Groups Working on Maize

Groups Working on Common Wheat

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Huw D. Jones /Rothamsted Research	Various varieties Florida (W) Fielder (S) Cadenza(S)	Agro,BioGun	600	RC,S	Tech dev, promoters, targeting/tagging/RNAi, seed/ endosperm/ cell wall development. Insect, fungal resistance
Pierre Barret /INRA	Bobwhite (S) Courtot (S) Apogee (S) Recital (W)	BioGun	400	RC	Yield, end-use quality, disease resistance, crossability
Dirk Becker /Biocentre Klein Flottbek, EBBT, University of Hamburgh	Florida (W)	BioGun	400	RC,T/H,S	Technology development, various applications
Paul Christou /Universitat de Lleida/ICREA	Bobwhite (S)	BioGun	200	RC	Mol pharming, stress metabolic engeneering, nutrient improvement,
Renato D'Ovidio /University of Tuscia	Bobwhite (S)	BioGun	25	RC,T/H	Cell wall protein inhibitors, storage proteins
Jochen Kumlehn /Leibniz Institute of Plant Genetics and Crop Plant Research	Bobwhite (S) Certo (W)	Agro	200	RC,T/H	Enabling technologies, Reproduction, stress, pathogens, pharming, development etc.
Emma Wallington /National institute for Agricultural Botany, Cambridge	Various varieties Glasgow (W) NB1 (S) Fielder (S)	Agro	3000	RC,S	Tech. dev., promoter and germplasm evaluation for cereal disease resistance, novel traits, pre-breeding targets
Francisco Barro Losada /Instituto de Agricultura Sostenible,	Bobwhite (S) Anza Perico	BioGun	50	RC,T/H,S	Cereal quality, exotic genes for cereal breeding
Henrik Brinch-Pedersen /University of Aarhus Preben Holm	Bobwhite (S)	BioGun	50	RC	Food/feed quality, technology development, cicgenesis, selection
Barnabás Jenes /Agricultural Biotechnology Center	CY-45 (S) GK Delibab (W) Dogankent	BioGun	100	RC,T/H,S	Biotic stress, promoters, organelle transformation
Laura Rossini /University of Milan Marcella Bracale	Bobwhite (S)	Agro,BioGun	100	RC	Stress tolerance, plant architecture
Zoltán Bedo /Agricultural Research Institute of the Hungarian Academy of Sciences Boglarka Kisgyorgy-Nemeth Annamaria Meszaros Klara Meszaros	Cadenza(S) Bobwhite (S) Mv Emese (W) Mv Toborzo (W) Mv Csárdás (W) Mv Mambo (W)	Agro,BioGun	25	RC	Technology development, silencing, dough strength, disease resistance
Beat Keller /Institute of Plant Biology, University of Zurich Silvia Travella	Bobwhite (S)	BioGun	40	RC,T/H	fungal disease resistance, RNAi. fluorescent tags
Mitic Nevena /Institute for Biological Research, "Sinia Stankovic"	Vesna, BL100 Norin 5 Tobarry 66 ZA205x-1	Agro	50	RC	Technology development, stress tolerance, herbicide resistance
János Pauk /Szeged	CY-45 (S)	BioGun	40	RC	Drought tolerance, rust resistance, gene function.
Eva Stoger /Vienna	Bobwhite (S)	BioGun	~50	RC	Mol Pharming, nutritional improvement, protein trafficking and storage.
Kamil Haliloglu /Ataturk University, Faculty of Agriculture	Fielder (S) Bobwhite (S)	Agro,BioGun	not available	RC	Abiotic stress, plastid transformation
Anna Nadolska-Orczyk /Plant Breeding and Acclimatization Institute	Torka Kontesa	Agro	50	RC	RNAi silencing, analysis of CKX, Pina Pinb genes
Christof Sautter /Institute of Plant Sciences, ETH Zurich	Frisal (S) Golin (S) Greina (S) Bobwhite (S)	BioGun	?		Fungal diseases, Iron content/ gut uptake
Laszlo Tamas /Eotvos Lorand University Biology Institute				RC	cereal quality , promoters, cereals as bioreactors, stress

Groups Working on Durum Wheat

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Renato D'Ovidio /University of Tuscia	Svevo	BioGun	100	RC,T/H	Cell wall protein inhibitors, storage proteins
Francisco Barro Losada /Instituto de Agricultura Sostenible,	Don Pedro Simeto, Vitron	BioGun	50	RC,T/H,S	Cereal quality, exotic genes for cereal breeding
Laura Rossini /University of Milan Marcella Bracale		Agro,BioGun	100	RC	Stress tolerance, plant architecture

Groups Working on Barley

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Dirk Becker /Biocentre Klein Flottbek, EBBT, University of Hamburgh	Igri	Agro	150	RC,T/H,S	Technology development, various applications
Henrik Brinch-Pedersen /University of Aarhus Preben Holm	Golden Promise Alexis Corniche Femina Salome	Agro,BioGun	90	RC	Food/feed quality, technology development, cicgenesis, selection
Wendy Harwood /John Innes Centre	Golden Promise	Agro	n100s	RC,T/H,S	Tech. dev. analysis of insertions, control of expression, safety
Jochen Kumlehn /Leibniz Institute of Plant Genetics and Crop Plant Research	Golden Promise Igri PF17048	Agro	>1000	RC,T/H	Enabling technologies, Reproduction, stress, pathogens, pharming, development etc.
Laura Rossini /University of Milan Marcella Bracale	Golden Promise	Agro	250	RC	Stress tolerance, plant architecture
Elana Todorovska /AgroBioInstitute Nabil Mosa Eyd Abu-Mhadi Helen Ougham	Ruen	BioGun	25		Expression of medical proteins and vaccines. Abiotic stress, overexpression and RNAi
Ralf. R. Mendel /Department of Plant Biology,Technische Universität Braunschweig	Golden Promise Salome	BioGun	50	RC	Molybdenum cofactor synthesis
Anna Nadolska-Orczyk /Plant Breeding and Acclimatization Institute	Scarlett Golden Promise	Agro	20	RC	RNAi silencing, analysis of CKX, Pina Pinb genes
Jennifer Stephens /James Hutton Institute - SCRI	Golden Promise	Agro	n100s	RC,T/H,S	Tech. dev. analysis of insertions, control of expression, safety
Laszlo Tamas /Eotvos Lorand University Biology Institute	Golden Promise	Agro	20	RC	cereal quality , promoters, cereals as bioreactors, stress

Groups Working on Oats

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Anna Nadolska-Orczyk /Plant Breeding and Acclimatization Institute	Slawko Akt Bajka	Agro	30	RC	RNAi silencing, analysis of CKX, Pina Pinb genes
Sue Dalton /Institute of Biological, Environmental & Rural Sciences			Can transform, details not given	RC	Forage quality, cell wall traits, apomixis etc

Groups Working on Rye

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Jochen Kumlehn /Leibniz Institute of Plant Genetics and Crop Plant Research	L22	Agro	<10	RC,T/H	Enabling technologies, Reproduction, stress, pathogens, pharming, development etc.

Groups Working on Tritordium

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Francisco Barro Losada /Instituto de Agricultura Sostenible,		BioGun	11	RC,T/H,S	Cereal quality, exotic genes for cereal breeding

Groups Working on Triticale

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Jochen Kumlehn /Leibniz Institute of Plant Genetics and Crop Plant Research	Bogo	Agro	<100	RC,T/H	Enabling technologies, Reproduction, stress, pathogens, pharming, development etc.
Anna Nadolska-Orczyk /Plant Breeding and Acclimatization Institute	Wanad	Agro	30	RC	RNAi silencing, analysis of CKX, Pina Pinb genes

Groups Working on Grasses

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Sue Dalton /Institute of Biological, Environmental & Rural Sciences	Lolium spp. Poa spp. Festuca spp. Agrotis spp.	Agro,BioGun	>400	RC	Forage quality, cell wall traits, apomixis etc

Groups Working on Brachypodium

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Philippe Vain /John Innes Centre	Bd21	Agro	2500	RC,T/H,S	Technology development, T-DNA tagging, clean-gene methods.
Sue Dalton /Institute of Biological, Environmental & Rural Sciences		Agro,BioGun		RC	Forage quality, cell wall traits, apomixis etc
Huw D. Jones /Rothamsted Research	Various varieties	Agro,BioGun	100	RC,S	Tech dev, promoters, targeting/tagging/RNAi, seed/ endosperm/ cell wall development. Insect, fungal resistance
Dirk Inze /Institute of Biological, Environmental & Rural Sciences			Under Development	D

Groups Working on Rice

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Peter Beyer /Albert-Ludwigs-Universität Freiburg	Taipei 309	Agro	700	D
Paul Christou /Universitat de Lleida/ICREA		BioGun	n1000s	RC	Mol pharming, stress metabolic engeneering, nutrient improvement,
Emmanuel Guiderdoni /CIRAD	Nipponbare ZongZou321 Azucena Moroberekan	Agro,BioGun	17000	RC,T/H,S	Root development, homologous recombination, insect resistance
Philippe Vain /John Innes Centre	Japonica spp. Javanica spp.	Agro	500	RC,T/H,S	Technology development, T-DNA tagging, clean-gene methods.
János Pauk /Szeged	Uggi 9	BioGun	40	RC	Drought tolerance, rust resistance, gene function.
Eva Stoger /Vienna	A 19	BioGun	~50	RC	Mol Pharming, nutritional improvement, protein trafficking and storage.
Christof Sautter /Institute of Plant Sciences, ETH Zurich	Taipei 309	Agro	?		Fungal diseases, Iron content/ gut uptake
Laszlo Tamas /Eotvos Lorand University Biology Institute	Taipei 309	BioGun	?	RC	cereal quality , promoters, cereals as bioreactors, stress

Groups Working on Maize

Scientist	Varieties	Methods	Output	Collaborations	Research Activities
		AGRObacterium tumefaciens or BIOlistics	Lines/Yr	conventional Research Collaborations; Training or Hotel facilities; costed transformation Service
Dirk Becker /Biocentre Klein Flottbek, EBBT, University of Hamburgh	Hi II H99 A188 B73	Agro,BioGun	1500	RC,T/H,S	Technology development, various applications
Paul Christou /Universitat de Lleida/ICREA	Various varieties M37W	BioGun	1000	RC	Mol pharming, stress metabolic engeneering, nutrient improvement,
Jochen Kumlehn /Leibniz Institute of Plant Genetics and Crop Plant Research	Hi II	Agro	300	RC,T/H	Enabling technologies, Reproduction, stress, pathogens, pharming, development etc.
Eva Stoger /Vienna	Hi II	BioGun	~50	RC	Mol Pharming, nutritional improvement, protein trafficking and storage.
Philippe Vain /John Innes Centre			Had success in maize in the past. Not currently transforming - could if required	RC,T/H,S	Technology development, T-DNA tagging, clean-gene methods.
Dirk Inze /Institute of Biological, Environmental & Rural Sciences			Protocol under development	D
Zoltán Bedo /Agricultural Research Institute of the Hungarian Academy of Sciences Boglarka Kisgyorgy-Nemeth Annamaria Meszaros Klara Meszaros	Various varieties		Protocol under development	RC	Technology development, silencing, dough strength, disease resistance
Elana Todorovska /AgroBioInstitute Nabil Mosa Eyd Abu-Mhadi Helen Ougham	Elite XM87-136	BioGun	25		Expression of medical proteins and vaccines. Abiotic stress, overexpression and RNAi

All the responses

Survey	Scientist	Institute	Department	Country
2	Pierre Barret	INRA	INRA GDEC (Génétique, Diversité et Ecophysiologie des Céréales)	France
3	Francisco Barro Losada	Instituto de Agricultura Sostenible,	Instituto de Agricultura Sostenible, CSIC	Spain
4	Dirk Becker	Biocentre Klein Flottbek, EBBT, University of Hamburgh	Biocentre Klein Flottbek, EBBT	Germany
5	Zoltán Bedo	Agricultural Research Institute of the Hungarian Academy of Sciences	Agricultural Research Institute of the Hungarian Academy of Sciences	Hungary
6	Peter Beyer	Albert-Ludwigs-Universität Freiburg	Biol. Inst II, Albert-Ludwigs-Universität Freiburg	Germany
8	Henrik Brinch-Pedersen	University of Aarhus	Department of Genetics and Biotechnology, Research Centre Flakkebjerg	Denmark
9	Paul Christou	Universitat de Lleida/ICREA	Universitat de Lleida/ICREA	Spain
11	Renato D'Ovidio	University of Tuscia	University of Tuscia	Italy
10	Sue Dalton	Institute of Biological, Environmental & Rural Sciences	Institute of Biological, Environmental and Rural Sciences	United Kingdom
13	Emmanuel Guiderdoni	CIRAD	Biological Systems Department Plant Development and Genetic Improvement (DAP) unit	France
14	Kamil Haliloglu	Ataturk University, Faculty of Agriculture	Faculty of Agriculture, Dept. of Field Crops	Turkey
15	Wendy Harwood	John Innes Centre	Department of Crop Genetics	United Kingdom
16	Dirk Inze	Institute of Biological, Environmental & Rural Sciences	VIB-Department of Plant Systems Biology	United Kingdom
17	Barnabás Jenes	Agricultural Biotechnology Center	Agricultural Biotechnology Center	Hungary
29	Huw D. Jones	Rothamsted Research	Plant Science Department	United Kingdom
18	Beat Keller	Institute of Plant Biology, University of Zurich	Institute of Plant Biology	Switzerland
19	Jochen Kumlehn	Leibniz Institute of Plant Genetics and Crop Plant Research	Leibniz Institute of Plant Genetics and Crop Plant Research	Germany
20	Ralf. R. Mendel	Department of Plant Biology,Technische Universität Braunschweig	Department of Plant Biology, Technische Universität Braunschweig	Germany
21	Anna Nadolska-Orczyk	Plant Breeding and Acclimatization Institute	Plant Breeding and Acclimatization Institute	Poland
22	Mitic Nevena	Institute for Biological Research, "Sinia Stankovic"	Department of Plant Physiology,	Serbia
23	János Pauk	Szeged	Cereal Research Non-profit Company	Hungary
7	Laura Rossini	University of Milan	University of Milan	Italy
25	Christof Sautter	Institute of Plant Sciences, ETH Zurich	Institute of Plant Sciences	Switzerland
24	Hans-Henning Steinbiss	Max-Planck-Institute for Plant Breeding Research	Max-Planck-Institute for Plant Breeding Research	Germany
36	Jennifer Stephens	James Hutton Institute - SCRI	Scottish Crop Research Institute	United Kingdom
26	Eva Stoger	Vienna	BOKU Vienna	Austria
27	Laszlo Tamas	Eotvos Lorand University Biology Institute	department of Plant Physiology and Molecular Plant Biology	Hungary
1	Elana Todorovska	AgroBioInstitute	Cereal functional genetics group AgroBioInstitute	Bulgaria
28	Philippe Vain	John Innes Centre	Department of Crop Genetics	United Kingdom
12	Emma Wallington	National institute for Agricultural Botany, Cambridge	Centre for Plant Genetics, Breeding and Evaluation	United Kingdom

Monogram Network Web Sites

Smart Carbohydrate Centre

The Smart Carbohydrate Centre provides completely new resources for end-users and breeders of wheat and barley, paving the way for the development of new cultivars with improved or novel food and industrial value, and of new, renewable raw materials for industry.

Wheat: the big picture

On this website we describe the life cycle of WHEAT, from seed germination to harvest ripe with special emphasis on the development of the grain after fertilisation.

Monogram news

24/01/2012 NEW
MONOGRAM 2012 Registration Form more ...

23/01/2012 NEW
PlantSci 2012 - the UK Plant Sciences Federation Inaugural Research Conference more ...

17/01/2012
Wheat Information Survey more ...

17/01/2012
Press Release: Breeding better grasses for food and fuel more ...

16/01/2012
Monogram Workshop 2012 more ...

04/01/2012
UK PlantSci 2012 - JIC more ...

28/11/2011
Centenary of the founding of the Plant Breeding Institute more ...

Monogram Vacancies

16/01/2012
Bristol: Lecturer/Senior Lecturer/Reader in Systems Biology Approaches to Plant Water Use more ...

16/01/2012
Bristol - Senior Lecturer/Reader in Biological Responses to Global Change more ...

06/01/2012
Senior Wheat Breeder - Paris more ...

06/01/2012
Senior Wheat Breeder - Suffolk more ...

Monogram web updates

Problems or suggestions for the web sites, please contact the Web administrator

The Biotechnology and Biological Sciences Research Council

Rothamsted Research provides no express or implied warranties of any kind, including but not limited to those of merchantability, fitness for a particular purpose and non-infringement of the proprietary rights, such as copyrights, patents and trade secrets, of third parties. Rothamsted Research makes every effort to ensure the quality and accuracy of the material on these web pages. However, it accepts no liability for the contents of these web pages, including any third party material.

MONOGRAM UK focus on grain and grass research